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SM Journal of Food and Nutritional Disorders

Effects of Sulfasalazine, Lactobacillus Plantarum (Lp-115) and Fish Oil in Experimental Colitis

[ ISSN : 2573-3664 ]

Abstract
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Received: 21-Aug-2015

Accepted: 12-Nov-2015

Published: 18-Nov-2015

Research Article | Volume 1 - Issue 1 | Article DOI :

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Paroschi TP1, Breganó JW2, Simão ANC2, Dichi I3* and Miglioranza LHS1

1Department of Food Science and Technology, University of Londrina, Brazil

2Department of Pathology, Clinical Analysis and Toxicology, University of Londrina, Brazil

3Department of Internal Medicine, University of Londrina, Brazil

Corresponding Author:

Dichi I, Department of Internal Medicine, University of Londrina, Rua Robert Koch no 60 Bairro Cervejaria, Londrina, Paraná, Brazil

Keywords

Inflammatory bowel disease; Probiotics; Nutritional therapy; Oxidative stress; Inflammation

Abstract

Ulcerative Colitis (UC) is a chronic inflammatory disease that affects the gastrointestinal tract, and studies have shown the association of inflammation and oxidative stress on its occurrence. The use of immunomodulatory nutrients and probiotics has resulted in remission phase prolongation and are considered a promising strategy for prevention and treatment of UC. The long chain n-3 fatty acids from fish oil have also shown favorable results in UC decreasing symptoms. This study aimed to evaluate n-3 fatty acids from fish oil and/or the probiotic Lactobacillus plantarum (Lp-115), combined with sulfasalazine, at inflammatory markers in Trinitro-Benzene Sulfonic Acid (TNBS)-induced colitis in male Wistar rats. The study lasted 21 days. Nutritional intervention was performed during all experiment and colitis induction was performed in the 14th day. The rats were divided into f ive groups: (A), control group; (B), sulfasalazine; (C), sulfasalazine with Lactobacillus plantarum fermented milk; (D), sulfasalazine with n-3 fatty acids and (E) sulfasalazine, n-3 fatty acids and Lactobacillus plantarum. All groups received water and food ad libitum and environmental conditions were controlled. Tissue samples were collected for Myeloperoxidase (MPO) determination. A Kruskal-Wallis test with Dunn’s post-test was applied to determine differences at level of 5 % significance (p<0.05) between groups. The results were demonstrated by reduction of tissue levels of myeloperoxidase. Beneficial effects were seen with the use of sulfasalazine with probiotic, but sulfasalazine association with n-3 fatty acids obtained results that were more favorable.

Citation

Paroschi TP, Breganó JW, Simão ANC, Dichi I and Miglioranza LHS. Effects of Sulfasalazine, Lactobacillus Plantarum (Lp-115) and Fish Oil in Experimental Colitis. SM J Food Nutri Disord. 2015; 1(1): 1005.