Keywords
Biodiversity management; Conservation; Genetic marker; Genetic repertoire; Methodology; Local resource manager; Parentage relationship; Sampling strategy; Sex ratio
Abstract
This study proposed a method for managing many diploid species together without constructing any statistical models for specific species. Our purpose was to estimate how many different genetic compositions exist in the adult generation involved in their reproduction (We referred this number as genetic repertoire) through our Molecular Mark and Recapture (MMR) method. First, we developed the MMR method for diploid species and proposed theoretical formulae to calculate the variance and confidence interval of the genetic repertoires. Second, we made three virtual diploid species (human or birds, harem-forming mammals, and plants), which included the first generation and the second generation, and then we conducted simulations to estimate the genetic repertoires of the first generation. Third, we showed a test study using microsatellite genotype data of wild boar. Our results showed that our methods would be useful, especially in tropical forests, because the method did not require highly sophisticated statistical models or much prior information for a species. Moreover, it was able to estimate the genetic repertoires with a one-time random sampling of the parent and offspring individuals. Furthermore, a decrease or increase in genetic repertoire would be detectable by increasing the number of random sample collections to twice or more. We consider it has great potential to enhance management methods of biodiversity by local people.
Citation
Murase K and Murase J. Estimation of Effective Population Size of Diploid Species Using the Molecular Mark and Recapture (MMR) Method. SM J Biometrics Biostat. 2017; 2(3): 1018.