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SM Journal of Biology

Importance of Molecular Testing of Hepatitis C Virus Infection in Tertiary Care Hospitals

[ ISSN : 2573-3710 ]

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Received: 19-Sep-2016

Accepted: 08-Nov-2016

Published: 08-Nov-2016

Research Article | Volume 2 - Issue 2 | Article DOI :

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Matam Kavitha1*, KalalIravathy Goud1, Adi Mahalakshmi1, Ravi Vempati1, Sargarika1, Madhuri1, Sunitha N2, Lavanya2, Abdulaziz A Alodhayani3 and Imran Ali Khan4

1Department of Molecular Biology and Cytogenetics, Apollo Hospitals, India

2Department of Infectious disease, Apollo Hospitals, India

3Department of Family and Community Medicine, College of Medicine, King Saud University, Saudi Arabia

4Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Saudi Arabia

Corresponding Author:

Matam Kavitha, Department of Molecular Biology and Cytogenetics, Apollo Hospitals, India

Keywords

Hepatitis C virus; Sequencing; Genotyping; Real time Polymerase chain reaction; Treatment; Prognosis

Abstract

The study shows the importance of currently available molecular diagnostic tests for detection, monitoring treatment and genotyping of the HCV, their clinical applications, and how these tests shed light on the natural history of HCV and the necessity of knowing the genotype for the treatment. To determine the distribution pattern of HCV genotypes in Hyderabad, South India and its time trend over last 6 years. The present study was undertaken in the Department of Molecular biology and Cytogenetics department, Apollo hospitals, Hyderabad from January 2010 to June 2016. The study was done on 300 patients. Real time PCR followed by nucleotide sequencing using automated nucleotide sequencer was used in HCV genotyping to understand the distribution pattern of HCV genotypes in Telangana. Among the 300 patients screened, 222(74%) were males, and 78(26%) are females. Among Genotype 1, 64(21.3%) were 1a, 53(17.6%) are 1b, 22(7.3%) are 1c. Genotype 2 in 19(6.3%). Among genotype 3, 31(10.3%) are Genotype 3a, 28(9.3%) are genotype 3b, 9(3%) are genotype 3h, 14 (4.6%) are genotype 3k. Among genotype 4, 33 are genotype 4a (11%), 24(8%) are genotype 4d, 4(1.3%) are genotype 5a. There were no reports of Genotype 6 in our study. A genotype test and quantitative HCV RNA test should be performed on all patients prior to therapy to best assess probability of response and to aid in selection of appropriate therapeutic regimen. Knowing the predominant genotypes is important to plan future prevention and treatment strategies as the treatment of different genotypes and prognosis differs considerably.

Citation

Kavitha M, Goud K, Mahalakshmi A, Vempati R, Sargarika, Madhuri, et al. Importance of Molecular Testing of Hepatitis C Virus Infection in Tertiary Care Hospitals. SM J Biol. 2016; 2(2): 1013.