Keywords
TBI; CSF; Blood; Bcl-2; P53; Histopathology
Abstract
The present study declares the relationship between the cause of death and postmortem alterations in some body fluids (CSF, serum and plasma). Immuno-histochemical and micro architecture examination of brain tissue of White New Zealand rabbits were applied at different Postmortem Interval (PMI) after Traumatic Brain Injury (TBI). Thirty adult male White New Zealand rabbits were divided into two groups; the first group was killed by cervical dislocation and the second through head trauma. Each group was subdivided into three times of PMIs (zero, 6, and 12 hrs PM). CSF was used to detect the levels of K+, Na+, Ca++, and albumin. While, lactic acid, hypoxanthine, ammonia and uric acid concentrations were measured in plasma. Estimation of High Mobility Group Box-1 (HMGB1), Interleukin-1-beta (Il-1B) and Tumor Necrosis Factor-alpha (TNF-α) were assessed in serum. In addition, immunohistochemical observations of Bcl2 and P53 apoptotic proteins in brain tissue. The results revealed that some of the examined markers as K+, Na+, albumin, ammonia, hypoxanthine and High Mobility Group Box-1(HMGB1) had the potential role in estimation of PMI at examined time periods in physical and traumatized death. Traumatic death induced severe cerebral hemorrhages and necrosis of cerebral parenchyma than physical death. Immunohistochemical results of P53 and Bcl-2 in brain tissue declared focal positive reactions of some neurons, astrocytes and microglia in different degrees with time since death. It was concluded that biochemical analysis of some body fluids, tissue pathological changes and apoptotic markers are applicable tools for assessing accurate PMI after traumatic brain injury and could have a crucial role in legal medicine.
Citation
Mohamed AAR, Elbohi KM, Sharkawy NIE and Hassan MA. Biochemical and Apoptotic Biomarkers as Indicators of Time Elapsed Since Death in Experimentally Induced Traumatic Brain Injury. SM J Forensic Res Criminol. 2017; 1(2): 1010.