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SM Tropical Medicine Journal

Molecular Profiling and Characterization of Integrons and Genotyping of Escherichia coli and Klebsiella pneumoniae Isolates Obtained from North Indian Tertiary Care Hospital

[ ISSN : 2573-363X ]

Abstract
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Received: 23-Nov-2015

Accepted: 28-Jan-2016

Published: 01-Feb-2016

Research Article | Volume 1 - Issue 1 | Article DOI :

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arrukh Sobia¹,², M Shahid¹,³, Sana Jamali¹,⁴, Haris M Khan¹, and Shahazad Niwazi²*

¹Department of Microbiology, J. N. Medical College and Hospital, India
²Faculty of Public Health and Tropical Medicine, Jazan University, Kingdom of Saudi Arabia
³Department of Microbiology, Immunology & Infectious Diseases, Arabian Gulf University, Kingdom of Bahrain
?Department of Microbiology, Integral Institute of Medical Sciences and Research, India

Corresponding Author:

Mónica C Botelho, INSA - National Institute of Health Dr. Ricardo Jorge, Rua Alexandre Herculano, Porto, Portugal, Email(s): monicabotelho@hotmail.com; monica.botelho@insa.min-saude.pt

Keywords

E. coli; K. Pneumoniae; Molecular Epidemiology; Integron; ?-Lactamase; Mobilizing Genetic Elements

Abstract

While the emergence of antibacterial drug resistance is a great emerging health issue that threatens the clinical usefulness of these drugs, it is important to study the spread of antibiotic resistance genes in order to understand the relationship between resistance gene pool and its mobilization through transposons and integrons. 125 cefoxitin-resistant E. coli (109) and K. pneumoniae (16) isolates were looked for the presence of integrons in order to illustrate the location of antibiotic resistance genes (especially blaampC ). The genotyping was done by RAPD so as to find out genetic relatedness among isolates. 55.20% (69/125) isolates were found positive for integrons. 41 isolates showed single amplification band for CS region, 20 showed two bands, 4 showed three bands and four isolates showed multiple banding patterns. Sul-1 was reported to be present in 3’CS, but we also observe 14/69 isolates that showed amplification for 5’CS-3’CS region but did not show presence of Sul-1 (when detected by PCR). Out of 109 E. coli isolates, 91 could be typed by RAPD, while 18 were found untypable. Among 91 E. coli isolates, 33 were grouped in 15 clusters while the remaining 58 isolates showed unique banding patterns indicating genetic un-relatedness. Among 16 K. pneumoniae isolates, 14 were typed by RAPD and 2 isolates were found untypable. The higher rate of resistance to several classes of β-lactam antibiotics in integron-positive isolates is probably attributable to the association of β-lactamase genes with integron-carrying plasmids and hence suggests that antibiotic drug resistance is transmitting through these mobilizing agents. As evident from RAPD-typing, most patients in our hospital were infected with different clades of organisms, thereby demonstrating clonal diversity among isolates suggesting horizontal transmission of bla genes

Citation

Sobia F, Shahid M, Jamali S, Khan HM and Niwazi S. Molecular Profiling and Characterization of Integrons and Genotyping of Escherichia coli and Klebsiella pneumoniae Isolates Obtained from North Indian Tertiary Care Hospital. SM Trop Med J. 2016; 1(1): 1003.