Abstract
Abstract The irradiation leads to delayed wound healing and the paradigms that can enhance the healing of irradiated wound will be useful, especially in conditions were wounds are exposed to ionizing radiation before or after surgery. The wound healing activity of various concentrations of hesperidin ointment was investigated in mice exposed to 6 Gy of whole body γ-radiation after infliction with a full thickness circular excision wound of 15 mm diameter by measuring wound contraction using videography and determining the mean wound healing time. Topical application of different concentrations of hesperidin ointment twice daily accelerated the healing of irradiated wounds in a concentration dependent fashion when compared to placebo application on the irradiated wounds. A maximum wound contraction was observed for 5% hesperidin ointment when compared to the other concentrations. Likewise, the mean wound healing time also reduced in a concentration dependent manner in the animals receiving a topical application of 5% hesperidin, where it was shorter by 2.7 days when compared to the irradiated placebo controls. The present study demonstrates that topical application of hesperidin accelerates healing of normal as well as irradiated wounds and may serve as a useful paradigm for the management of irradiated wounds in a clinical setting and after nuclear disaster. The acute toxicity determination of hesperidin showed that animals were able to tolerate 5 g/kg body weight and it was no observed adverse effect level (NOAEL) dose. No symptoms of toxicity in any form were observed at this dose. In vitro free radical scavenging revealed that hesperidin scavenged, DPPH, hydroxyl, superoxide, ABTS+ and Nitric oxide radical in a concentration dependent manner.
Citation
Jagetia GC and Rao KVNM. Topical Application of Hesperidin, a Citrus Bioflavanone Accelerates Healing of Full Thickness Dermal Excision Wounds in Mice Exposed to 6 Gy of Whole Body Γ-Radiation. SM J Nutr Metab. 2017; 3(2): 1021.