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SM Journal of Biology

Distribution of Acetylated Histone 4 in Normal Liver and Acetaminophen Induced Liver Damage

[ ISSN : 2573-3710 ]

Abstract
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Received: 23-Dec-2016

Accepted: 01-Feb-2017

Published: 06-Feb-2017

Research Article | Volume 3 - Issue 1 | Article DOI :

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Nakamura N1*, Chang C-W2, Yang X1, Shi Q1, Salminen WF1 and Suzuki A3,4

1Division of Systems Biology, National Center for Toxicological Research, Food and Drug Administration, United States

2Division of Bioinformatics and Biostatistics, National Center for Toxicological Research, Food and Drug Administration, United States

3Division of Gastroenterology, Central Arkansas Veterans Healthcare System, United States

4Division of Gastroenterology, University of Arkansas for Medical Sciences, United States

Corresponding Author:

Nakamura N, Division of Systems Biology, National Center for Toxicological Research, Food and Drug Administration, United States, Tel: +1-870-543-7175

Keywords

Acetaminophen; Liver; Mice; Necrosis; Histone acetylation

Abstract

To describe cell-specific acetylation of histones and their zonal localization in normal mouse liver and characterize their changes in acetaminophen (APAP)-damaged mouse liver 24 hr after APAP exposure, we performed an immunohistochemical study using normal and APAP-injured mouse livers. Cell-specific expression of acetylated H4 was observed in normal mouse liver. The highest acetylated H4 expression levels were found in bile duct epithelial cells, followed by sinusoidal non-parenchymal cells, venous endothelial cells, and hepatocytes. Zonal differences were observed; acetylated H4 expression was stronger in zone 1 (periportal) than in zone 3 (pericentral). In APAP-treated mouse liver, acetylated H4 expression decreased as tissue damage developed, and was significantly negatively correlated with tissue damage and alanine aminotransferase levels (p<0.05). Acetylated H4 expression in sinusoidal non-parenchymal cells did not decrease after tissue damage. The method implemented herein can measure acetylated H4 expression in a cell- and zone-specific manner and aid future investigation on pathobiological relevance of altered histone modification in liver injury and repair.

Citation

Nakamura N, Chang C-W, Yang X, Shi Q, Salminen WF and Suzuki A. Distribution of Acetylated Histone 4 in Normal Liver and Acetaminophen-Induced Liver Damage. SM J Biol. 2017; 3(1): 1014.