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A novel, simple and economic Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the quantification of Amoxicillin (AMX) in bulk and tablet dosage form with greater precision and accuracy. Separation was achieved on Hypersil ODS C18 (250mm×4.6×5micron) column in isocratic mode with mobile phase consisting of Acetonitrile: 0.2M Potassium dihydrogen phosphate buffer (pH 5) (1:99v/v) and conditions optimized with flow rate of 1 ml/minute and wavelength of detection at 254 nm. The retention time of Amoxicillin (AMX) was found to be 6.992 min. The method was validated as per ICH guidelines. Linearity was established for Amoxicillin (AMX) in the range0.6 – 3.4 μg / ml with R2 value 1. The percentage recovery of Amoxicillin (AMX) was found to be in the range 98.87-99.87 %. The high recovery and low relative standard deviation confirm the suitability of the proposed method for the estimation of the drug in bulk and marketed dosage form. Validation studies demonstrated that the proposed RP-HPLC method is simple, specific, rapid, reliable and reproducible for the determination of Amoxicillin for Quality Control level.

Pigments are widely distributed in sugarcane plant cells and have been associated with color in sugarcane juice for white sugar production. Chlorophylls, phenolics and flavonoids compounds are some of those pigments. Mechanized harvesting increases the adding of cane tops to processing and the study of these compounds allows us to understand their impact on the final product. The aim was to separate and quantify these compounds obtained from sugarcane top ethanolic extracts. The chromatographic occurred in adsorption resin. The quantification was made in spectrophotometric systems. These methods allow separation and quantification of compounds and even observation of polarities of molecules compared to the eluents.

The purpose of the study was to isolate and identified the alkaloids fractions local plants in Malaysia. TLC of alkaloid extracts from the plants used in this study revealed the presence of these compounds by using Dragendroff’s reagent to reveal characteristic orange bands of alkaloids. IR spectra of alkaloids extract from the fruit of M. citrifolia, leaves of A. squamosa, and the roots of A. Angustiloba exhibited a strong O-H from the fruit of M. citrifolia. C-H stretching groups are shown for the fruit of M. citrifolia, the leaves of A. squamosa as well as the roots A. angustiloba. The N-H groups are showed in the fruit of M. citrifolia and the root A. angustiloba. The C=O bond at the leaves of A. squamosa and the roots of A. angustiloba. C-H group bonds were detected for the fruit of M. citrifolia, the leaves of A. squamosa and the roots of A. angustiloba. It could be concluded that the alkaloids of the plants can be a new source of antimicrobials against pathogenic bacteria and antioxidant source.

Cereal samples fortified with folic acid obtained in the metropolitan area of Monterrey were analyzed by a chromatographic method developed in our laboratory. The results were compared with the product´s label nutritional information. All tested cereal samples matched the folate labeled. The chromatographic method applied is quick, simple, low cost and suitable for cereal folic acid determination.