Investigation of Carcinoma basocellulare solidum exulcerans, Carcinoma basocellulare superficial multicentricum, and Trichoepithelioma was performed using histology and biomineralogical methods. Obtained data confirmed elevated levels of some elements in altered skin tissues. Moreover, rare micrograins of phosphates were observed. Additionally, examination of biomineralization of human tissues suggests that higher local mineralization (of tissue fluids) may lead to mistakes in DNA code at the moment of cell division. It is possible that cancer tissues are secondarily mineralized by activity of cancer cells. Further research is needed to answer questions that arose.

The article presents the analysis of the clinical picture of patients with rosacea of different degrees of severity complicated with demodicosis. The dermatovenereology life quality index was evaluated for patients with rosacea in the presence and absence of Demodex mites. Presence of Demodex mites reduces the life quality of patients with rosacea. The duration of pathological processes on the facial skin, as well as the number of relapses were assessed. The course of rosacea was analyzed depending on the species identity of Demodex mites (Demodex folliculorum and Demodex folliculorum brevis). In the structure of rosacea diagnoses Demodex folliculorum is significantly more prevalent (n = 20; 66.7%) then Demodex folliculorum brevis (n=8; 26.7).

Malassezia species are part of the normal mycobiota of skin of animals and humans but they can cause skin and blood stream infections as well. These yeasts are all lipid dependent explained by the absence of fatty acid synthase genes in their genome. At the same time, metabolic reconstruction revealed differences in the metabolism of fungal steroids and degradation of CoA-activated long-chain FAs, arachidonic acid, and butanoate metabolism between Malassezia yeasts. In addition, differences in the assimilation of palmitic acid were predicted. Indeed, M. furfur was able to metabolize palmitic acid but M. globosa, M. sympodialis, M. pachydermatis, and an atypical variant of M. furfur were not able to do so. Tools to genetically modify Malassezia have become available recently, which will speed up the process to decipher mechanisms underlying growth and pathogenicity of these yeasts. Here, we will provide an overview about the genus Malassezia and make an assessments to the new insights in this yeast.

Background: Skin grafting is a vital part of the treatment of deep skin defects. The preferred thickness of the graft is determined on the parameters of the wound which needs to be covered. Although, it is not known in how far the dial setting of the dermatome corresponds with the actual thickness of the harvested skin graft. To determine the usability of a dermatome setting regarding the resulting grafts thickness.

Methods: During the harvesting of split skin grafts in the operation theatre, the average thickness of harvested skin grafts was compared to the dermatome thickness settings.

Results: A total of 50 grafts were collected from 30 patients, leading to 250 thickness measurements (5 per graft). The measured thickness differed significantly from the dermatome setting: the grafts were up to 50% thinner than the dial setting when the settings of 0.25 mm and 0.30 mm were used. Scalp harvest thickness varied from 50% thinner to 200% thicker than the setting. Thickness was more or less constant across the skin grafts both lengthwise and laterally. We did not detect an effect of the blade becoming blunt regarding its accuracy after repeated use.

Conclusion: These results imply that the average measured skin graft thickness is up to 50% thinner than the thickness setting. Graft shrinkage alone does not seem to pose an adequate explanation for this difference. In scalp harvests, there is more variability in the thickness of the grafts. Furthermore, our results suggest that grafting blades do not have to be changed during the procedure, because we found no signs of bluntness.

Stomatitis with involvement of the caudal area is a severe inflammation of the oral tissues in cats, which heavily impairs the animal health. The affected gingiva and mucous membrane show different degrees of inflammation, proliferation and ulceration. The only way to manage this serious disease is suppression of inflammation, but often reduction of inflammation at tolerable levels is considered a satisfactory therapeutic result. Many different treatments have been proposed, but currently a therapy of choice and a standardized treatment for stomatitis management have not yet identified. Therefore, new therapeutic strategies are needed to effectively counteract inflammation and improve symptoms.

Laser therapy has been applied with significant results in the management of human patients affected by Oral Mucositis (OM), where it has been effective in reducing inflammation, pain, severity and duration of OM ulcers. The present paper reports the results of a preliminary proof-of-concept clinical study aimed at verifying the hypothesis that NIR laser therapy could be usefully applied to treat cat mucositis.

The enrolled patients were divided into two groups, in order to compare two treatments differing for the energy density released. The effectiveness of laser therapy in reducing inflammation and favoring ulcer healing was assessed by clinical examination of patients, histological analysis and Immunofluorescence microscopy on tissue specimens collected from the animals before and after the cycle of laser therapy.

The outcomes of this preliminary study demonstrate that laser therapy with suitable treatment parameters can be effective in the management of stomatitis.

Even if with different response times in the two groups, laser therapy induced an improvement of the symptoms that was greater and faster in the group treated with lower energy density.