Background: Toxoplasmosis is a cosmopolitan disease with acute and chronic infections, caused by the obligate intracellular protozoan parasite Toxoplasma gondii that can infect a variety of cells in almost all warm blooded animals including humans.

The study aimed to determine the seroprevalence of T. gondii infection among males in Duhok city using ELISA (IgG and IgM).

The relationship between toxoplasmosis and reproductive hormones including testosterone, free testosterone, and Follicle Stimulating Hormone (FSH) levels and its association with male sterility were also investigated.