Keywords
Siemens ADVIA 2120i; Bone marrow; Flow cytometry; Hematology analyzer; Automated cytochemistry
Abstract
Background: Bone marrow examination plays a central role in clinical management of many primitive and secondary hematological diseases. We conducted a systematic evaluation of Siemens ADVIA2120i analytical performance pertaining to automated analysis of bone marrow aspirate fluids.
Methods: 88 samples of bone marrow fluids were analyzed with ADVIA2120i and compared with a flow cytometry assay for total nucleated cells and manual microscopy for differential counts.
Results: Regarding total nucleated cells, ADVIA2120i showed a strong and significant correlation with flow cytometric counts, with a positive systematic bias due to interference by lipid particles. The myeloperoxidase reaction allowed ADVIA2120i to obtain a strong and significant correlation with the elements of the neutrophilic lineage. The same was observed between myeloperoxidase negative events in the PEROX channel and manual counts of NRBCs, lymphocytes and plasma cells. Moreover, scatter-plots were found to be an important source of information for the study of bone marrow aspirates.
Conclusion: Automated cytometric analysis with ADVIA 2120i could be potentially useful for bone marrow examination
Citation
Canovi S, Galli V, Bergonzini G and Campioli D. Automated Analysis of Bone Marrow Aspiration Fluid Using Siemens ADVIA 2120i Hematology Analyzer. SM J Clin Pathol. 2017; 2(1): 1008