Article Details

Neuroprotective Effect of Organic and Conventional White Grape Juice against Carbon Tetrachloride Damage in Different Brain Areas of Rats

[ ISSN : 2573-6728 ]

Abstract

Clarice M. Peripolli, Tatiane Gabardo, Fernanda de Souza Machado, Mariane Wohlenberg, Juliana D.O. Lima, Alice S. Oliveira, Marina Rocha Frusciante, Niara da Silva Medeiros, Sheila Pereira Feijó, Filipe V.V. Nascimento, Caroline Dani, and Cláudia Funchal

Centro Universitário Metodista - IPA, Porto Alegre, RS, Brazil

Corresponding Author:

Cláudia Funchal, Centro Universitário Metodista – IPA, Porto Alegre – RS, Brazil; Telephone: 5551 3316 1233; E-mail: claudia.funchal@metodistadosul. edu.br; csfunchal@yahoo.com.br

Keywords: Oxidative stress; Antioxidants; Grape juice; Neuroprotection; Brain

Abstract

The consumption of nutrients containing phenolic compounds has been reported due to the benefits they produce on human health. Therefore, the objective of this study was to investigate the antioxidant and neuroprotective effect of the administration of organic (OGJ) and conventional (CGJ) white grape juices from Niagara variety on the oxidative stress in cerebral cortex, hippocampus and cerebellum after the treatment with carbon tetrachloride (CCl4 ) as well as on some biochemical parameters in serum of rats. Adult male rats (~300g; n=6-8/group) were orally treated (gavage) with 7μL/g of OGJ, CGJ or water, for a period of 14 days. On the 15th day it was administered CCl4 (3.0mL/kg). After 4h the animals were euthanized and the cerebral cortex, hippocampus and cerebellum were dissected and used for the analysis of oxidative stress parameters. We observed that CCl4 enhanced lipid peroxidation (TBARS) and protein damage (carbonyl), reduced the nonenzymatic antioxidants defenses (sulfhydryl), and changed the activity of the enzymatic antioxidants defenses catalase (CAT), Superoxide Dismutase (SOD) in the brain of rats. CCl4 also enhanced glucose, Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST) and Gamma-Glutamyl (GGT) and decreased total cholesterol and High-Density Lipoprotein (HDL) in serum of rats. CGJ and OGJ were able to prevent or ameliorate most of these alterations. Consequently, regular intake of white grape juice could be considered as an adjuvant in the therapy of oxidative damages, revealing a possible antioxidant and neuroprotective agent.

Citation

Peripolli CM, Gabardo T, Machado FS, Wohlenberg M, Lima JDO, Oliveira AS, et al. Neuroprotective Effect of Organic and Conventional White Grape Juice against Carbon Tetrachloride Damage in Different Brain Areas of Rats. SM J Neurol Neurosci. 2017; 1(1): 1006.

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Received: 10-Jan-2017

Accepted: 11-Mar-2017

Published: 15-Mar-2017