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SM Journal of Biomedical Engineering

The Effect of WIN55, 212-2 on Protein S100, Matrix Metalloproteinase-2 and Nitric Oxide Expression of Chondrocyte Monolayer

[ ISSN : 2573-3702 ]

Abstract
Details

Received: 03-Oct-2016

Accepted: 03-Jan-2017

Published: 06-Jan-2017

Research Article | Volume 3 - Issue 1 | Article DOI :

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A Abdeldayem¹, M Youseffi¹*, F Sefat¹, M Genedy¹, MM Abdul Jamil², and F Javid³

¹Faculty of Engineering and Informatics, School of Engineering-Medical and Healthcare Technology, University of Bradford, UK
²Department of Electronic Engineering, Faculty of Electrical and Electronic Engineering, Universiti Tun Hussein Onn, Malaysia
³Pharmacy, School of Applied Sciences, University of Huddersfield, Queensgate, Huddersfield, UK

Corresponding Author:

M Youseffi, Faculty of Engineering and Informatics, School of Engineering Medical and Healthcare Technology, University of Bradford, UK, Email: m.youseffi@bradford.ac.uk

Keywords

Chondrocyte; WIN55, 212-2; Wound healing; Protein expression; Collagen type-I and II; Fibronectin; MMP 2 and NO expression

Abstract

Studies have been conducted to highlight the anti-inflammatory and immunosuppressive properties of synthetic cannabinoids as well as their potential for cartilage repair. Various wound healing techniques can be used to investigate the mechanisms of chondrocyte repair in monolayers or three dimensional tissues constructs. In this work the effect of WIN55, 212-2 (WIN-2) on nitric oxide (NO) and matrix metalloproteinase-2 (MMP-2) expressed by wounded chondrocyte monolayers was investigated. Moreover, expression of collagen type-I and type-II, fibronectin and S100 proteins were detected using immunofluorescence and quantitatively verified using ELISA based techniques following treatment with 1 µM and 2 µM of WIN-2. Treating chondrocytes with 1 µM of WIN-2 significantly increased expression of collagen type-II, fibronectin and S100, and significantly reduced collagen type-I expressions as compared to the control groups. On the other hand, both concentrations of WIN-2 significantly reduced the expression of the inflammation markers NO and MMP-2 in a dose dependent manner. These findings highlight the potential use of the synthetic cannabinoids for improving cartilage healing properties as well as acting as an anti-inflammatory agent which could be used to enhance tissue engineering protocols aimed at cartilage repair.

Citation

Abdeldayem A, Youseffi M, Sefat F, Genedy M, Jamil MMA and Javid F. The Effect of WIN55, 212-2 on Protein S100, Matrix Metalloproteinase-2 and Nitric Oxide Expression of Chondrocyte Monolayer. SM J Biomed Eng. 2017; 3(1): 1011.