Pintomyia fischeri adults collected in different eco-epidemiological studies in the northeastern of Argentina were found parasitized by juvenile nematodes (Tylenchida) isolated from the body cavity. The percentage of infected females and males was 3.8% and 2.9% respectively. Part of the life cycle of sand flies and tylenchid nematodes take place in humid and dark sites, where infection of immature stage of Phlebotominae insects is possible. Biology of this parasite could help to determine the breeding sites of sand flies. This study constituted the first report of tylenchid nematodes infecting sand flies at field conditions in South-America.

We hypothesize that the level of various biomolecules is variable and remain in a dynamic state during the course of development in Gastrothylax crumenifer. The aim of the present study is to determine whether these molecules are in dynamic state or their levels are constant in all the developmental stages. The present study was designed to investigate level of various macromolecules e.g. protein, glycogen, lipid, lipid fractions and nucleic acids by standard Spectrophotometric methods. All the biomacromolecules are expressed in terms of mg/g wet weight. A minimum of three separate replicates were run for each experiment. To investigate similarities/ differences in polypeptides of different developmental stages namely fresh eggs, eggs containing mature miracidia (Em), cercariae, metacercariae, immature and adult stages, SDS-gradient PAGE was performed. In the present study we have investigated biochemical alterations during the larval stages of Gastrothylax crumenifer (a rumen infecting paramphistome) which revealed marked differences during the miracidial development as well as in the intra-molluscan larval stages. We observed that the protein, glycogen and lipid of freshly laid eggs were utilized during the development. Concentration of protein, glycogen and lipid contents significantly elevated in the cercariae as compared to the non-feeding metacercarial stage, indicating an adaptation of the parasite to build up the nutrients for the metacercariae which is a dormant stage. Very little amount of lipid was detected in the fresh eggs (E0) which further decreased in the eggs containing mature miracidia (Em) and then increased sharply in the cercarial and metacercarial stages indicating the substantial buildup of lipid reserves. Nucleic acid contents decreased from E0 to the subsequent developmental stages. Such changes could be of intrinsic significance for the cellular differentiation and organogenesis in larval paramphistomes. Further, we analyzed polypeptide profile of developmental stages which revealed heterogeneous mixture of polypeptides. A total of 27, 15, 14, 15, 14 and 34 polypeptides in E0, Em, cercariae, metacercariae, immature and adult G. crumenifer, respectively were resolved. Eight and nine characteristic polypeptides (17-90 kDa) were observed in E0 and adult stages respectively.

While the emergence of antibacterial drug resistance is a great emerging health issue that threatens the clinical usefulness of these drugs, it is important to study the spread of antibiotic resistance genes in order to understand the relationship between resistance gene pool and its mobilization through transposons and integrons. 125 cefoxitin-resistant E. coli (109) and K. pneumoniae (16) isolates were looked for the presence of integrons in order to illustrate the location of antibiotic resistance genes (especially blaampC ). The genotyping was done by RAPD so as to find out genetic relatedness among isolates. 55.20% (69/125) isolates were found positive for integrons. 41 isolates showed single amplification band for CS region, 20 showed two bands, 4 showed three bands and four isolates showed multiple banding patterns. Sul-1 was reported to be present in 3’CS, but we also observe 14/69 isolates that showed amplification for 5’CS-3’CS region but did not show presence of Sul-1 (when detected by PCR). Out of 109 E. coli isolates, 91 could be typed by RAPD, while 18 were found untypable. Among 91 E. coli isolates, 33 were grouped in 15 clusters while the remaining 58 isolates showed unique banding patterns indicating genetic un-relatedness. Among 16 K. pneumoniae isolates, 14 were typed by RAPD and 2 isolates were found untypable. The higher rate of resistance to several classes of β-lactam antibiotics in integron-positive isolates is probably attributable to the association of β-lactamase genes with integron-carrying plasmids and hence suggests that antibiotic drug resistance is transmitting through these mobilizing agents. As evident from RAPD-typing, most patients in our hospital were infected with different clades of organisms, thereby demonstrating clonal diversity among isolates suggesting horizontal transmission of bla genes

In a recent issue of Cancer Letters Gouveia and colleagues [1] studied a series of 40 Angolan patients diagnosed with Urogenital Schistosomiasis (UGS).

The rapid rise in the number of reported clinical cases of Zika in South and Central America over the span of a few months in late 2015 and early 2016 presents a real risk of a global epidemic of this Aedes mosquito transmitted viral infection. This threat is exacerbated by the globalisation of the human population which promotes the movement of asymptomatic carriers between nations in the same or separate continents. Pregnant women are considered at highest risk since microcephaly in the developing foetus is strongly associated with, if not yet proven as caused by, Zika infection. The possibility of additional modes of virus transmission, vertical and sexual, if confirmed, suggests the life cycle of the virus to be very complex and thus limits the scope of predictive models, which in turn may impede control efforts. Currently, no anti-Zika vaccine is available and while this has now been prioritised by multiple funding agencies, it may take several years to come to commercial fruition. The fact that Zika is closely related to yellow fever and Japanese encephalitis viruses, for both of which effective vaccines exist, offers hope that the fast-tracked preparation of a candidate vaccine is feasible. However, performing clinical trials on pregnant women would provide ethical and practical challenges to overcome before licensure is granted for administration to the general public. In the meantime, alternative public health management strategies, such as vector control programs to target mosquito breeding, are required in order to limit the global spread of this re-emerging disease.