Di(2-ethylhexyl) phthalate (DEHP) is recognized as an endocrine-disrupting compound and is widely adopted as a plasticizer. Evidence links its reproductive toxicity to the tumor suppressor PTEN, genomic stability is crucially maintained by DNA damage repair (DDR). PTEN loss promotes DNA damage accumulation and cellular senescence. Resveratrol (RES), a natural polyphenol, exhibits antioxidant and anti-inflammatory activities and shows protective potential for the male urogenital system. Nevertheless, the mechanisms associated with testicular senescence due to DEHP exposure and RES protection remain incompletely understood. This study employed in vivo and in vitro models, including cell viability assays, testosterone ELISA, cell cycle distribution analysis, senescence marker staining, Western blotting, and PTEN modulation. DEHP exposure significantly reduced testosterone levels in rat testis and TM3 cells, which was ameliorated by RES. DEHP/MEHP treatment upregulated senescence markers (β-Gal, p21, p16, γH2AX), downregulated PTEN and CDK2, and increased p-CHK2. These changes were reversed by RES. PTEN overexpression attenuated MEHP-induced senescence, G1 arrest, and testosterone reduction, while PTEN inhibition abolished RES protection. These findings elucidate the mechanism by which RES protects against DEHP-induced senescence via inhibiting PTEN loss.

Lung squamous cell carcinoma (LUSC) is one of the most common subtypes of non-small cell lung cancer, accounting for approximately 25-30%. Chemotherapy remains one of the main treatment options for lung squamous cell carcinoma, but long-term use often leads to the development of drug resistance in LUSC, resulting in reduced chemotherapy efficacy. This report described a patient with lung squamous cell carcinoma who underwent albumin paclitaxel+carboplatin chemotherapy after being diagnosed with right primary bronchogenic carcinoma (LUSC, cT3N1M0 stage IIIA). Two months later, examination revealed that the tumor volume seemed to have increased, indicating the development of chemotherapy resistance. Phosphorylation omics analysis was performed on adjacent and tumor tissues of patients, and it was found that the phosphorylation abundance of some loci in genes such as TRAF2 (T7) and PAK2 (S2) changed, which may be a key factor in the development of chemotherapy resistance.

Background/Objectives: Conventional psychometric instruments generally show limited ability to correlate with circulating biological markers. ADNe® is a 29-item psychogenic instrument based on a neurotransmitter-weighted response framework, designed to model functional patterns of neurobiological activation rather than classify personality traits. This study aimed to explore whether ADNe® neurofactor scores are associated with plasma concentrations of cortisol, interleukin-6 (IL-6), and testosterone in healthy adults.

Methods: Twenty healthy volunteers (15 women, 5 men; mean age 30.3 ± 9.6 years, range 22–61) completed the ADNe® questionnaire and provided a peripheral blood sample on the same day. Plasma was isolated by centrifugation (400 × g, 5 min), and biomarker levels were quantified using ELISA. Statistical analyses included Spearman rank correlations and exploratory multiple linear regression models using the seven neurofactors as simultaneous predictors.

Results: Significant associations were identified between selected neurofactors and biomarkers. Sm (attentional amplitude) showed an inverse correlation with testosterone (ρ = −0.567; p = 0.022), while Mn (strategic acuity) was inversely associated with IL-6 (ρ = −0.489; p = 0.046). Exploratory multivariate models showed high apparent explanatory capacity within this dataset (R² = 0.848 for IL-6, 0.735 for testosterone, and 0.569 for cortisol). However, given the small sample size and the number of predictors included, these estimates should be interpreted with caution.

Conclusions: In this pilot study, ADNe® neurofactor profiles showed associations with circulating biomarkers, suggesting that this approach may capture dimensions related to physiological regulation. Nevertheless, the findings are exploratory and may be influenced by overfitting and un-measured confounding variables. Independent replication in larger, pre registered studies with appropriate validation strategies will be necessary to confirm the robustness and generalizability of these results.