
Enterococcus saccharolyticus Es 3/11 D27 Isolated from Horses and its Postbiotic Activity
The species Enterococcus saccharolyticus is rarely occurred and identified. No information exists about this species strain bacteriocin/bioactive/postbiotic potential which could be utilized e.g. for bacterial prevention/protection. Standard microbiological methods as well as PCR analyses were used to basic characterization the strain 3/11D27. To test bacteriocin/ postbiotic potential, agar spot test was conducted and 158 indicator bacteria (147 Gram-positive and 11 Gram-negative). The strain E. saccharolyticus Es 3/11D27 was isolated from the inner part of the auricle mucosa of clinically healthy mare (Slovak breed Norik from Murá?) using sequence analysis. After sequencing, the strain was involved in GenBank with accession number MN822909. This strain has produced bacteriocin substance with inhibitory potential against Gram-positive and Gram-negative indicator bacteria non- depending on the indicator bacteria species. The inhibitory activity reached 100 up to 800 AU/ml. Es 3/11D27 has been also susceptible to antibiotics tested. It has been hemolysis-, deoxyribonuclease- negative with absence of virulence factors genes. Low-grade biofilm-forming ability using the plate assay was found in Es 3/11D27. It is first time presenting horses-derived species strain E. saccharolyticus with bacteriocin activity. Additional studies will be processed to determine detail characteristic of bacteriocin substance produced by the strain Es 3/11D27 with the aim for its further application potential in horses breeding.
Andrea Lauková1*, Eva Styková2 ,Valentína Focková1 and Marián Maďar2