JSM Veterinary Medicine and Research

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Enterococcus saccharolyticus Es 3/11 D27 Isolated from Horses and its Postbiotic Activity

The species Enterococcus saccharolyticus is rarely occurred and identified. No information exists about this species strain bacteriocin/bioactive/postbiotic potential which could be utilized e.g. for bacterial prevention/protection. Standard microbiological methods as well as PCR analyses were used to basic characterization the strain 3/11D27. To test bacteriocin/ postbiotic potential, agar spot test was conducted and 158 indicator bacteria (147 Gram-positive and 11 Gram-negative). The strain E. saccharolyticus Es 3/11D27 was isolated from the inner part of the auricle mucosa of clinically healthy mare (Slovak breed Norik from Murá?) using sequence analysis. After sequencing, the strain was involved in GenBank with accession number MN822909. This strain has produced bacteriocin substance with inhibitory potential against Gram-positive and Gram-negative indicator bacteria non- depending on the indicator bacteria species. The inhibitory activity reached 100 up to 800 AU/ml. Es 3/11D27 has been also susceptible to antibiotics tested. It has been hemolysis-, deoxyribonuclease- negative with absence of virulence factors genes. Low-grade biofilm-forming ability using the plate assay was found in Es 3/11D27. It is first time presenting horses-derived species strain E. saccharolyticus with bacteriocin activity. Additional studies will be processed to determine detail characteristic of bacteriocin substance produced by the strain Es 3/11D27 with the aim for its further application potential in horses breeding.

Andrea Lauková1*, Eva Styková2 ,Valentína Focková1 and Marián Maďar2


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Characterization of Qualitative Morphological Traits in Indigenous Goats in Ethiopia Using Multivariate Analysis

Characterization of goat breeds based on morphological trait variations is essential for planning breed improvement programs and conservation strategies. This study aimed to use multiple correspondence analysis to evaluate qualitative morphological traits and identify traits that best describe the morphological structure of Hararghe goats. The results of the study show that there are significant differences in the distribution of some qualitative morphological traits among the three districts (Bedeno, Fedis, and Gorogutu) while others do not show significant differences. The first principal axis explained 15% of the principal inertia, the second principal axis explained 12%, and cumulatively the first two principal axes explained 27% of the principal inertia. The MCA method identified systematic relationships among traits and trait attributes. On the dimensions identified, the sample goat population of Fedis and Gorogutu clustered together with concave head profile, straight horn shape, obliquely upward horn orientation, presence of Toggle, and pigmented Muzzle; while the corresponding values of goats in Bedeno clustered together with the presence of horn, curved and spiral horn shape, backward and lateral horn and ear orientation with slope down from wither, slope up toward rump, and pendulous. These findings provide valuable information for better multivariate characterization and conservation of indigenous goat ecotypes.

Kefelegn Kebede*, Bushra Mohammad, Mengistu Urge and Ashenafi Getachew Megersa


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Case Report: Nutritional Management of Canine Renal Insufficiency Utilizing a Home-Cooked Diet and Supplementation

Renal insufficiency is difficult to diagnose in the early stages due to delayed clinical presentation and significant blood value changes. However, dysfunction in the kidneys can lead to life-threatening changes in physiology. When renal insufficiency is suspected, it is important to utilize nutrition as a mode of intervention. This case report follows a canine patient aging between 13 to 17, reporting clinical changes and blood renal values after administration of an NSAID and possible escalation of renal burden. The case outlines an example of utilizing integrative veterinary medicine practices including a home cooked diet plan with supplementation to address signs of renal insufficiency and gastric ulceration, resulting in complete resolution of clinical signs including symptoms and blood measures.

Debbie Decker¹, Samantha Koziol²*


Latest Articles

Enterococcus saccharolyticus Es 3/11 D27 Isolated from Horses and its Postbiotic Activity

Andrea Lauková1*, Eva Styková2 ,Valentína Focková1 and Marián Maďar2

Pages: 11

Characterization of Qualitative Morphological Traits in Indigenous Goats in Ethiopia Using Multivariate Analysis

Kefelegn Kebede*, Bushra Mohammad, Mengistu Urge and Ashenafi Getachew Megersa

Pages: 11

Case Report: Nutritional Management of Canine Renal Insufficiency Utilizing a Home-Cooked Diet and Supplementation

Debbie Decker¹, Samantha Koziol²*

Pages: 11

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Seth J. Worley, MD, FHRS, FACC

Director, Interventional Implant Program MedStar Heart & Vascular Institute, Washington, DC, USA

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